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Evaluation of Sedative activity of Cannabis Sativa in mice

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  • Samuel Okenwa,
  • Jane Chidera Nwachukwu,
  • Chisom Peace Ezeala ,
  • Ifeanyi Eze,
  • Emmanuel Buchi Onyioha,
  • Nnamdi Umuokeke Amarachi ,
  • Austine Nkemakolam Okorie ,
  • Lazarus Chukwuemeka Ejike,
  • Uzoigwe Delight Ihechukwu
Samuel Okenwa
Department of Pharmaceutical and medicinal Chemistry, University of Nigeria, Nsukka Pharmaceutical Sciences, University of Nigeria, Nsukka, Enugu,
Jane Chidera Nwachukwu
College of Medicine, University of Nigeria Teaching Hospital- Enugu,
Chisom Peace Ezeala
Department of Pharmaceutical and medicinal Chemistry, University of Nigeria, Nsukka Pharmaceutical Sciences, University of Nigeria, Nsukka, Enugu
Ifeanyi Eze
3Faculty of pharmaceutical sciences, Enugu State University of Science and Technology
Emmanuel Buchi Onyioha
Department of Pharmaceutical and medicinal Chemistry, University of Nigeria, Nsukka Pharmaceutical Sciences, University of Nigeria, Nsukka, Enugu
Nnamdi Umuokeke Amarachi
Department of Pharmaceutical and medicinal Chemistry, University of Nigeria, Nsukka Pharmaceutical Sciences, University of Nigeria, Nsukka, Enugu,
Austine Nkemakolam Okorie
Department of Pharmacology and Toxicology, University of Nigeria, Nsukka
Lazarus Chukwuemeka Ejike
Department of Pharmaceutical and medicinal Chemistry, University of Nigeria, Nsukka Pharmaceutical Sciences, University of Nigeria, Nsukka, Enugu,
Uzoigwe Delight Ihechukwu
Department of Pure and Industrial Chemistry, University of Nigeria, Nsukka.
Published in : International Journal of Scientific Research and Management (IJSRM) , Vol. 12 No. 11 (2024)

Submission to VIJ 2024-11-08

Keywords

  • Cannabis sativa Linn,
  • Neuropharmacological,
  • phytochemical,
  • Sedative,
  • Elevated plus-maze

Copyright (c) 2024 Samuel Okenwa, Jane Chidera Nwachukwu, Chisom Peace Ezeala , Ifeanyi Eze, Emmanuel Buchi Onyioha, Nnamdi Umuokeke Amarachi , Austine Nkemakolam Okorie , Lazarus Chukwuemeka Ejike, Uzoigwe Delight Ihechukwu

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

Background: Cannabis sativa Linn. (Family: Cannabaceae) is commonly cultivated in Bangladesh in moist areas such as riverbanks, lakes, canals, and roadsides. The leaves of this plant are traditionally used to treat insomnia, depression, and neurodegenerative diseases. 

Objective: The study's objective is to investigate the sedative effects and phytochemical composition of the Cannabis sativa species in Nigeria.

Methods: The elevated plus maze was used to assess the sedative effect of various fractions of aqueous methanol extracts of Cannabis sativa at a dosage of 100 mg/kg in mice. Comparisons were made between the sedative action of the extract and diazepam, which was used as the positive control. Additionally, acute toxicity was also evaluated during the assessment.

Results: During the elevated plus-maze test, various parameters such as the number of entries in open arms and the number of entries in closed arms were recorded. The administration of different fractions of aqueous methanol extracts of Cannabis sativa resulted in a significant increase (p < 0.001) in the time spent in and the number of entries into open arms compared to the control group.

Conclusion: The findings suggest that various concentrations of water-methanol extracts from Cannabis sativa exhibit sedative effects that are superior to those of the control (diazepam), supporting the use of this plant in traditional medicine. Further research should be conducted to investigate the active components of Cannabis sativa and understand the mechanism of its effects before proceeding to clinical trials.

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