The Use of Eco-Enzyme as a Larvacide for Aedes aegypti Mosquito Larvae

Abstract

Nearly half of the world's population is at risk of dengue infection, an arboviral infection that is typically spread by the mosquito Aedes aegypti. Insecticide use and community awareness campaigns are the main vector control strategies currently in use in some countries, and they didn’t done many ways to reduce dengue. Eco-enzyme is the product of fermentation from organic waste such as vegetable, fruit, and sugar. Eco-enzyme can be used in agriculture, medicine, and others. This study aims to analyze the toxicity level of ecoenzyme as the larvicide for Aedes aegypti larvae. An analytical experimental study were conducted in Bionas Parasitology Laboratory from February to March 2024. As many as 20 Aedes aegypti larvae were used in each of exposure. Each exposure were replicated four times. The  mortality  of Aedes  aegypti larvae  was analyzed  by a  two-way ANOVA  test, followed  by  a  Fisher's least significant defference (LSD) post  hoc  test  with  a  significance  level  of (p<0.01). This   data   was analysed by  Statistical  Product  and  Service Solutions(SPSS)Inc. 25.0. The concentrations of ecoenzyme induce mortality of the third instar of Aedes aegypti larvae were started from 2% concentration, and the highest was found in 10% concentrations which resulted in average of 50% mortality. In this context, the analysis results show that time, concentration, and the interaction between time and concentration have a significant influence on the observed quantities. These findings can have important implications in the context of studies or experiments related to the variables being tested. Below are the profile plots of each variable (concentration and time of exposure) which give significant influence on the mortality of Aedes larvae